产品货号：
GS2185
中文名称：
PspPPI内切酶
英文名称：
PspPPI
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?
技术规格
相关搜索：PspPPI内切酶，PspPPI限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA(dcm-,HindIII-digest)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | RG↑GWCCY |
| 来源 | Pseudomonas species PP |
| Assayed on | Lambda DNA(dcm-,HindIII-digest) |
| Optimal buffer | Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);200 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.|Store at -20℃.? |
| Ligations | After 5-fold overdigestion with enzyme more than 70%of the DNA fragments can be ligated and more than 80%of these can be recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Blocked by overlapping Dcm methylation(CmCWGG):RGGWCCTGG. |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | PpuMI,Psp5II |
相关搜索：PspPPI内切酶，PspPPI限制性内切酶